Analytical Chemistry Progress by Robert B. Green (auth.)

By Robert B. Green (auth.)

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Their results seem to suggest that the band considered was to be attributed mainly to a higher dye concentration inside than outside the band. 3 General Remarks Further experiments about DNA are discussed in Chapter 5. As pointed out in Chapter 2 UV-light may cause lesions (like pyrimidine dimers) in DNA. An error made in the cell during the repair of damaged DNA could impact 36 Laser Spectroscopyof Biomolecules the first step towards carcinogenesis s4~. Therefore, the study of the molecular basis of UV radiation mutagenesis will remain an important area of interest in photobiology.

Shultens, H. , Hellweg, M. , Jovin, T. : Appl. Opt. 18, 303 (1979) 24. Ware, B. R. : in ref. [5], Vol. 2, pp. 199 25. , Rigler, R. : in Tunable Lasers and Applications (ed. ), Heidelberg: Springer 1976 26. Melamed, M. , Mullaney, P. F~, Mendelsohn, M. L. ): Flow Cytometry and Sorting, New York: Wiley 1979 27. Horan, P. , Wheeles, L. L. : Science 198, 149 (1977) 28. : Appl. Phys. 18, 177 (1979) 29. Campillo, A. , Shapiro, S. : in ref. [20], pp. 364 30. Parson, W. : in ref [5], Vol. I, pp. 339 31.

G. streak cameras which enable the time-resolved detection of very short pulses. ). Secondly, they can be irradiated' in living cells. In order to irradiate special sites inside a single cell, a laser beam is connected to a microscope (microbeam systems) 15,16). g. the "secondary fluorescence" of the bound dye molecules if the biomolecules absorb only in the UV or if they have an extremely low quantum yield of fluorescence. Thirdly, questions of photo28 Laser Spectroscopyof Biomolecules Table2.

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